SV40 Immortalized Human Dermal Microvascular Endothelial Cells
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Product Overview
Description
SV40 Immortalized Human Dermal Microvascular Endothelial Cells (IM-HDMEC) are a stable, continuously proliferating cell line derived by Immortalizing Primary Human Dermal Microvascular Endothelial Cells (HMEC) with the Simian Virus 40 (SV40) Large T antigen.
The SV40 Large T antigen drives immortalization by suppressing the tumor suppressor gene products p53 and retinoblastoma (Rb), thereby bypassing replicative senescence and enabling indefinite cell proliferation in vitro.
IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.
Key Features
Catalog Number: NBP0005-ICSV40
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
CELLS CHARACTERIZATION
1. Cytoplasmic VWF/Factor VIII >95% positive by immunofluorescence.
2. Cytoplasmic uptake of Di-I-Ac-LDL >95% positive by immunofluorescence.
3. Cytoplasmic PECAM1 >95% positive by immunofluorescence.
4. HDMVECs are negative for HIV-1, HBV, HCV, and mycoplasma.
Quick Facts
Product Type
Immortalized Cells
Viability
>95% viable (Trypan Blue exclusion)
Applications
* Studies of dermal vascular physiology and patho…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
* Studies of dermal vascular physiology and pathophysiology * Angiogenesis and tube formation assays * Inflammation and leukocyte-endothelial cell interaction studies * Lipoprotein-endothelium interaction studies * Drug screening and toxicity testing * High Throughput Screening (HTS) and High Content Screening (HCS) assays * Investigation of endothelial barrier function and permeability
Suitable for * studies of dermal vascular physiology and pathophysiology * angiogenesis and tube formation assays * inflammation and leukocyte-endothelial cell interaction studies * lipoprotein-endothelium interaction studies * drug screening and toxicity testing * high throughput screening (hts) and high content screening (hcs) assays * investigation of endothelial barrier function and permeability studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
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