GCaMP6m-Expressing Human Umbilical Vein Endothelial Cells
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Product Overview
Description
GCaMP6m is stably introduced into Human Umbilical Vein Endothelial cells (HUVECs) through lentiviral transduction. Lentiviral particles that carry the GCaMP6m transgene facilitate stable genomic integration, allowing for persistent and heritable expression of the calcium indicator across multiple cell passages. To ensure uniform expression of GCaMP6m, stably transduced cells can be selected and enriched using antibiotic resistance methods (such as puromycin or hygromycin) and/or fluorescence-activated cell sorting (FACS).
Key Features
Catalog Number: NBP-0001-GCaMP6m
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
CELLS CHARACTERIZATION
HUVECs grow as a cobblestone-like monolayer in culture and are characterized by the expression of endothelial markers including von Willebrand Factor (vWF/Factor VIII) and CD31 (PECAM-1).
Quick Facts
Product Type
Human Endothelial Cells
Viability
>98% viable (Trypan Blue exclusion)
Applications
* Live-cell fluorescence microscopy and time-laps…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
* Live-cell fluorescence microscopy and time-lapse calcium imaging. * High-content screening of vasoactive compounds
Suitable for * live-cell fluorescence microscopy and time-lapse calcium imaging. * high-content screening of vasoactive compounds studies and research applications
drugs
Suitable for drugs studies and research applications
and biologics. * Study of mechanosensitive Ca2+ channels (e.g.
Suitable for and biologics. * study of mechanosensitive ca2+ channels (e.g. studies and research applications
Piezo1) in endothelial mechanobiology. * Investigation of intercellular Ca2+ wave propagation in endothelial monolayers. * Organ-on-a-chip and microfluidic vascular models. * Drug toxicity and cardiotoxicity screening platforms. * Co-culture models with smooth muscle cells
Suitable for piezo1) in endothelial mechanobiology. * investigation of intercellular ca2+ wave propagation in endothelial monolayers. * organ-on-a-chip and microfluidic vascular models. * drug toxicity and cardiotoxicity screening platforms. * co-culture models with smooth muscle cells studies and research applications
pericytes
Suitable for pericytes studies and research applications
or immune cells.
Suitable for or immune cells. studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
Technical Support
Our experts are available for technical questions and protocol assistance.
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