Human Umbilical Vein Endothelial Cells (HUVEC)

HUVECs (Human Umbilical Vein Endothelial Cells, catalogue number (NBP-0001) are expertly isolated from pooled, healthy human umbilical veins, making them a crucial cellular model for investigating endothelial function and related diseases. These cells serve as a cornerstone in vascular biology research due to their pivotal role in a variety of physiological and pathological processes, including angiogenesis, inflammation, and blood vessel function.

Upon shipping, HUVECs are carefully provided in frozen vials at passage 1. This early passage is specifically chosen to ensure the maximum vitality and performance of the cells when cultured, thereby supporting optimum results in experimental applications. To facilitate proper growth and maintenance of these cells, it is recommended to use Endothelial Growth Medium (EGM; catalogue number NBP-02), which is rich in nutrients, containing 10% serum along with essential growth supplements. Under the recommended culture conditions, HUVECs can achieve a minimum average population doubling level exceeding 20, providing a reliable and reproducible cell source for experimental use.

CULTURE REQUIREMENTS

Component Recommendation
Basal Medium : Endothelial Basal Medium (EBM)
Growth Supplement : Endothelial Growth Supplement
Serum : 10% Fetal Bovine Serum (FBS)
Coating Matrix Required: NeoCoating Solution
Culture Vessel : Pre-coated tissue culture plastic
Incubation : 37°C, 5% CO, 95% humidity
Split Ratio : 1:2 to 1:4 every 3-5 days
Maximum Passages : Recommended: P2-P6


IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.