Adipogenic Maintenance Medium
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Product Overview
Description
CIHM-MED4 is a sterile, complete, serum-containing medium formulated to support the maintenance and terminal differentiation of Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (CIHM001) along the adipogenic lineage. This medium is intended for use after the doxycycline withdrawal phase (using CIHM-MED2) to allow SV40 LT expression to shut down, enabling the cells to exit proliferation and undergo stable adipogenic differentiation.
The medium promotes the accumulation of intracellular lipid droplets, which can be visualized by Oil Red O staining, and supports long-term maintenance of mature adipocytes.
Note: This medium does not contain doxycycline or puromycin.
Note: This medium contains tetracycline-free FBS to ensure no inadvertent activation of the TetON system during differentiation.
Key Features
Catalag Number: CIHM-MED4
Size: 500ml
Appearance: Clear, pink to slightly red solution
pH: 7.2 – 7.4 (at 25°C)
Osmolality: 310 – 350 mOsm/kg
Sterility: 0.1 µm filtered, sterile
Endotoxin level: < 0.5 EU/mL
IMPORTANT: This warranty is valid for one month from the original purchase date.
Quick Facts
Product Type
Cells Culture Media
Viability
High viability
Applications
Research & Development
Availability
In Stock • Global Shipping
Technical Specifications
Applications & Use Cases
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
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