Doxycycline Withdrawal Medium
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Product Overview
Description
Doxycycline Withdrawal Medium (CIHM-MED2) is a complete, ready-to-use culture medium formulated specifically for the controlled growth arrest of Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (Catalog#CIHM001). These cells express SV40 Large T antigen (SV40 LT) under a TetON-inducible promoter.
The presence of doxycycline drives proliferation; its withdrawal silences SV40 LT expression, reverting cells to a quiescent, primary like state.
This medium contains no doxycycline and no puromycin, enabling the cessation of transgene expression and allowing differentiation studies or long-term quiescent culture. It retains all basal nutrients and growth supplements required for cell viability and lineage-specific differentiation.
IMPORTANT: This warranty is valid for one month from the original purchase date.
Key Features
Catalag Number:CIHM-MED2
Size: 500ml
Light sensitivity: Protect from prolonged light exposure (no doxycycline, but medium contains light-sensitive vitamins)
Shelf life: 6 months from the date of manufacture when stored properly.
Pre-use preparation: No additional supplementation required; warm to 37°C before use
Color: Pink to reddish-pink (phenol red indicator)
Note: Do not freeze the medium; freezing causes precipitation of salts and loss of
functionality.
Quick Facts
Product Type
Cells Culture Media
Viability
High viability
Applications
Research & Development
Availability
In Stock • Global Shipping
Technical Specifications
Applications & Use Cases
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
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