Osteogenic Induction Medium
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Product Overview
Description
CIHM-MED2B is a sterile, complete osteogenic induction medium specifically formulated to drive differentiation of Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (Catalog #CIHM001) toward the osteoblast lineage.
This medium contains a standardized formulation of ascorbic acid (ascorbate), glycerophosphate, and dexamethasone, the three essential components for osteogenic
differentiation.
For optimal results, CIHMSCs must first be cultured in CIHM-MED2 (Doxycycline Withdrawal Medium) for 48–72 hours to silence SV40 Large T antigen expression before switching to CIHM-MED2B. This ensures terminal differentiation without
uncontrolled proliferation.
Differentiated cells demonstrate mineralized extracellular matrix, detectable by Alizarin Red S or von Kossa staining, and express osteoblast-specific markers, including alkaline
phosphatase (ALP), osteocalcin (OCN), and runt-related transcription factor 2 (RUNX2).
Note: This medium is serum-containing (10% FBS) and does NOT contain doxycycline or puromycin. It is intended for use after transgene silencing.
Key Features
Catalog Number: CIHM-MED2B
Size: 500ml
Appearance: Clear, pink to slightly orange-red solution
pH 7.2 – 7.4 (at 25°C)
Osmolality: 300 – 340 mOsm/kg
Sterility: 0.1 µm filtered, sterile
IMPORTANT: This warranty is valid for one month from the original purchase date.
Quick Facts
Product Type
Cells Culture Media
Viability
High viability
Applications
Research & Development
Availability
In Stock • Global Shipping
Technical Specifications
Applications & Use Cases
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
Technical Support
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