Human Brain Microvascular Endothelial Cells (HBMVECs)
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Product Overview
Description
Human Brain Microvascular Endothelial Cells (HBMVECs) are the primary cellular constituents of the blood brain barrier (BBB), a highly selective and dynamic interface between the peripheral blood circulation and the central nervous system (CNS). The BBB tightly controls the exchanges between the blood and brain compartments, protecting and maintaining the delicate interstitial environment that is optimal for neuronal communication.
HBMVECs are morphologically and functionally distinct from endothelial cells found in peripheral vasculature. These differences confer the brain’s vasculature with a uniquely restrictive and regulated barrier function, making HBMVECs indispensable both physiologically and as research tools.
HBMVECs are typically derived from microvessels isolated from temporal lobe tissue removed during operative treatment of epilepsy. The tissue is mechanically fragmented and size filtered using polyester meshes, and the resulting microvessel fragments are placed onto type I collagen-coated flasks to allow HBMVECs to migrate and proliferate.
Guarantee expansion for a minimum of 2-5 population
doublings.
IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.
Key Features
Catalog Number: NBP-0002
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO
CELL CHARACTERISTICS
*Structural and Morphological Features:
- Absence of fenestrations: Unlike peripheral endothelium, brain endothelial cells lack fenestrations, which minimizes unregulated paracellular transport.
- Minimal pinocytic activity: HBMVECs exhibit a low level of pinocytic vesicles, reducing non-specific transcellular transport.
* Molecular Markers:
-von Willebrand Factor (vWF): A classic endothelial cell marker.
-CD31 (PECAM-1): A cell adhesion molecule expressed on endothelial cells.
- VE-Cadherin: An adherens junction protein critical for vascular integrity.
-Glucose Transporter-1 (GLUT-1): Facilitates glucose transport across the BBB.
- P-glycoprotein (P-gp): An efflux pump that actively expels xenobiotics and drugs from the brain.
-Caveolin-1: Associated with caveolae-mediated transcytosis.
Quick Facts
Product Type
Human Endothelial cells
Viability
>98% viable (Trypan Blue exclusion)
Applications
-In Vitro Blood-Brain Barrier Modeling HBMVECs…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
-In Vitro Blood-Brain Barrier Modeling HBMVECs are the gold standard cell type for constructing in vitro BBB models. Their monolayers exhibit high TEER and have proven useful in multiple functional studies for in vitro modeling of the human BBB. These models are used to: * Study BBB permeability and transport mechanisms. * Evaluate the effects of inflammatory stimuli
Suitable for -in vitro blood-brain barrier modeling hbmvecs are the gold standard cell type for constructing in vitro bbb models. their monolayers exhibit high teer and have proven useful in multiple functional studies for in vitro modeling of the human bbb. these models are used to: * study bbb permeability and transport mechanisms. * evaluate the effects of inflammatory stimuli studies and research applications
toxins
Suitable for toxins studies and research applications
and pathogens on barrier integrity. * Assess barrier disruption and recovery in response to pharmacological agents. Primary cultures of HBMVECs are highly pure (97% endothelial cells; 3% pericytes)
Suitable for and pathogens on barrier integrity. * assess barrier disruption and recovery in response to pharmacological agents. primary cultures of hbmvecs are highly pure (97% endothelial cells; 3% pericytes) studies and research applications
reproducible
Suitable for reproducible studies and research applications
and display characteristic brain endothelial markers
Suitable for and display characteristic brain endothelial markers studies and research applications
robust expression of tight and adherens junction proteins
Suitable for robust expression of tight and adherens junction proteins studies and research applications
caveolin-1
Suitable for caveolin-1 studies and research applications
and efflux protein P-glycoprotein. - CNS Drug Delivery Research One of the major limitations in the development of drugs for CNS diseases is the inability of most therapeutic molecules to cross the BBB. HBMVECs are widely used to evaluate and optimize drug delivery strategies
Suitable for and efflux protein p-glycoprotein. - cns drug delivery research one of the major limitations in the development of drugs for cns diseases is the inability of most therapeutic molecules to cross the bbb. hbmvecs are widely used to evaluate and optimize drug delivery strategies studies and research applications
including: * Nanoparticle-based delivery systems: Brain uptake of nanoparticle-loaded drugs can be enhanced by conjugation with ligands targeting specific proteins at the surface of brain microvascular endothelial cells. * Efflux pump studies: P-glycoprotein and other efflux transporters expressed by HBMVECs are major obstacles to CNS drug delivery and are extensively studied using these cells. * Transcytosis pathways: HBMVECs are used to investigate receptor-mediated transcytosis
Suitable for including: * nanoparticle-based delivery systems: brain uptake of nanoparticle-loaded drugs can be enhanced by conjugation with ligands targeting specific proteins at the surface of brain microvascular endothelial cells. * efflux pump studies: p-glycoprotein and other efflux transporters expressed by hbmvecs are major obstacles to cns drug delivery and are extensively studied using these cells. * transcytosis pathways: hbmvecs are used to investigate receptor-mediated transcytosis studies and research applications
a key mechanism for delivering macromolecules across the BBB.
Suitable for a key mechanism for delivering macromolecules across the bbb. studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
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