Cre-Expressing Human Primary Bronchial Fibroblasts
Quick Actions
Documentation 0 files
Product Overview
Description
Cre-Expressing Human Primary Bronchial Fibroblasts are a specialized cell line developed for groundbreaking research in respiratory biology, gene manipulation, and the intricate pathways involved in cellular signaling.
These fibroblasts serve as an invaluable resource for investigating the specific functions of targeted genes in lung tissue, allowing for a deeper understanding of their contributions to various pulmonary diseases.
IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.
Key Features
Catalog Number: NBP-1988Cre
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
CELL CHARACTERISTICS
Origin: These cells are derived from human primary bronchial tissue, carefully harvested from biobanked samples to ensure high quality and relevance to human physiology.
- Genetic Modification: Engineered to express Cre recombinase, enabling researchers to perform precise conditional gene knockout experiments through the Cre-loxP system. This feature facilitates the temporal and spatial control of gene deletion, crucial for understanding gene function in vivo.
- Morphology: Exhibiting a fibroblast-like morphology, they are characterized by elongated, spindle-shaped cells that adhere firmly to tissue culture plastic, allowing for efficient proliferation.
- Growth Conditions: Cells thrive in a humidified incubator maintained at 37°C with 5% CO2, mimicking physiological conditions for optimal growth.
- Media Recommendations: Cultivation is best achieved in fibroblast growth medium supplemented with 10% fetal bovine serum (FBS) and additional growth factors tailored to promote fibroblast viability and function.
Quick Facts
Product Type
Cre-Expressing Human Cells
Viability
>95% viable (Trypan Blue exclusion)
Applications
Gene editing through Cre-loxP system for targeted…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
Gene editing through Cre-loxP system for targeted gene modification. - Studying fibroblast response to growth factors
Suitable for gene editing through cre-loxp system for targeted gene modification. - studying fibroblast response to growth factors studies and research applications
cytokines
Suitable for cytokines studies and research applications
and mechanical stress. - Investigating the role of fibroblasts in pulmonary diseases such as asthma
Suitable for and mechanical stress. - investigating the role of fibroblasts in pulmonary diseases such as asthma studies and research applications
fibrosis
Suitable for fibrosis studies and research applications
and COPD.
Suitable for and copd. studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
Technical Support
Our experts are available for technical questions and protocol assistance.
Contact Support
