Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (CIHMSCs)
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Product Overview
Description
Conditionally Immortalized Human Bone Marrow-Derived Mesenchymal Stem Cells (CIHMSCs) are generated from primary human BM-MSCs via lentiviral transduction with
a TetON-inducible SV40 Large T antigen (SV40 LT) expression system.
The cells exhibit typical MSC morphology: spindle-shaped, fibroblast-like cells. Proliferation is controlled by doxycycline; in the presence of doxycycline, SV40 LT is expressed, driving proliferation. Upon doxycycline withdrawal, SV40 LT expression ceases, and cells return to a quiescent, primary-like state.
Cells were selected with puromycin to ensure stable integration and expression of the transgene. These cells retain key MSC surface marker expression and multilineage differentiation potential under non-permissive conditions.
IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.
Key Features
Catalag Number: CIHM001
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:2 (P2)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
Quick Facts
Product Type
Stem Cells
Viability
>80% viable (Trypan Blue exclusion)
Applications
Research & Development
Availability
In Stock • Global Shipping
Technical Specifications
Applications & Use Cases
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
Technical Support
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