Trypsin/EDTA Solution (0.05%)
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Product Overview
Description
Trypsin/EDTA Solution (0.05%) (NBPTE-03) is a sterile, readytouse cell dissociation reagent formulated for gentle detachment of adherent cells from tissue culture surfaces.
The solution contains porcine trypsin (0.05%) complexed with EDTA (0.53 mM) in phosphatebuffered saline (PBS). The EDTA chelates divalent cations (Ca², Mg²) that are required for cellsubstrate and cellcell adhesion, enhancing the proteolytic activity of trypsin. This product is optimized for routine subculturing of endothelial cells as well as
many other adherent cell types.
Intended Use: For research use only. Not for human or clinical applications.
Key Features
Catalog Number: NBPTE-03
Form: Liquid, sterile
Volume: 100 mL
Trypsin Concentration: 0.05% (w/v) (500 mg/L)
EDTA Concentration: 0.53 mM (approx. 0.02% w/v)
Buffer: Phosphate buffered saline (PBS), without Ca², Mg²
pH: 7.2 – 7.4 (at 25°C)
Osmolality: 280 – 320 mOsm/kg
Endotoxin Level: 0.5 EU/mL
Sterility: Negative for bacteria, fungi, and mycoplasma
Storage : –20 °C (or 2–8 °C
Shelf Life: 12 months when stored at –20 °C
IMPORTANT: This warranty is valid for one month from the original purchase date.
Quick Facts
Product Type
Primary cells
Viability
High viability
Applications
-Routine passaging of adherent cell lines and pri…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
-Routine passaging of adherent cell lines and primary cells -Dissociation of endothelial cells
Suitable for -routine passaging of adherent cell lines and primary cells -dissociation of endothelial cells studies and research applications
fibroblasts
Suitable for fibroblasts studies and research applications
epithelial cells
Suitable for epithelial cells studies and research applications
and many other cell types. -Harvesting cells for cryopreservation or downstream assays -Preparation of single cell suspensions for flow cytometry
Suitable for and many other cell types. -harvesting cells for cryopreservation or downstream assays -preparation of single cell suspensions for flow cytometry studies and research applications
sorting
Suitable for sorting studies and research applications
or molecular biology applications
Suitable for or molecular biology applications studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
Technical Support
Our experts are available for technical questions and protocol assistance.
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