Advancing CNS Drug Discovery with the NEO3DHB-6 3D Human Blood–Brain Barrier Model

Advancing CNS Drug Discovery with the NEO3DHB-6 3D Human Blood–Brain Barrier Model

By Dr. James Whitfield

The blood–brain barrier (BBB) remains one of the most formidable challenges in central nervous system drug development. Its highly selective nature protects the brain but also prevents the vast majority of therapeutics from reaching their targets. To bridge this gap, researchers need predictive, human-relevant in vitro tools—and that’s exactly what the NeoBioPharma 3D Human Blood–Brain Barrier Model (NEO3DHB-6) delivers.

A Physiologically Relevant Co-Culture System

The NEO3DHB-6 is an advanced in vitro co-culture system that mirrors the key structural and functional properties of the human BBB. Constructed with human brain microvascular endothelial cells, human brain pericytes, and human brain astrocytes, this insert-based 3D model supports endothelial polarization, tight junction formation, and robust barrier function. The result is a differentiated, high-value platform that closely recapitulates in vivo physiology—giving you data you can trust.

Designed for Versatile Research Applications

Whether you’re investigating CNS drug permeability, neuroinflammation, barrier integrity, or neurotoxicity, the NEO3DHB-6 is built to handle it. Key applications include:

• Drug BBB permeability assays and CNS drug delivery studies

• Blood-to-brain transport and transporter activity research

• BBB toxicology and brain endothelial toxicity assays

• Disease modeling and cell–cell interaction studies

• Tight junction protein expression and localization

• Receptor-mediated transcytosis and transport pathway modulation

• Microbial pathogenesis and neuroimmune target screening

Ready When You Are

The NEO3DHB-6 comes in a convenient 6-well insert-based format and is shipped frozen for stable, long-term storage at -80°C. The recommended experimental window is Day 4 to Day 7 after thawing and activation, ensuring optimal performance for your assays. With global shipping, technical support, and full product documentation available, NeoBioPharma makes it easy to integrate this model into your workflow.

Accelerate Your CNS Research

The NEO3DHB-6 is more than a model; it's a platform for discovery. By providing a human-relevant, 3D co-culture system, it empowers researchers to make better decisions earlier in the drug development pipeline. Whether you're screening novel compounds or unraveling the mechanisms of neurovascular disease, this model offers the physiological relevance and reproducibility you need.

Learn More

Explore the NEO3DHB-6 and the full range of 3D tissue models at neobiopharma.com. For technical questions or to request documentation, contact the NeoBioPharma team at info@neobiopharma.com.