Conditionally Immortalized Human Adipose Tissue-Derived Mesenchymal Stem Cells

1. Regenerative Medicine: Due to their ability to secrete large amounts of angiogenic factors (VEGF HGF FGF EGF IGF) and immunomodulatory cytokines (IL-6 IL-8) these cells are being evaluated for wound healing tissue repair and vascularization therapies. 2. Cartilage and Bone Engineering: Modified lines (e.g. SOX9-overexpressing ASC52telo) have demonstrated enhanced chondrogenic potential and responsiveness to mechanical and inflammatory stimuli providing platforms for studying joint disease and drug screening. 3. In Vitro Disease Modeling: Standardized immortalized lines reduce donor-to-donor variability and cell heterogeneity that complicate primary cell studies enabling reproducible experimental models. 4. Cell Tracking: Fluorescently labeled immortalized hAT-MSCs can be employed in in vivo cell-tracking experiments expanding their utility in preclinical studies. 5. Conditioned Medium Therapy: The secretome of these cells rich in growth factors and cytokines can be harvested as conditioned medium offering a cell-free alternative to direct transplantation with reduced risks associated with heterologous cell therapy.