Human Umbilical Vein Endothelial Cells (HUVECs) with Aequorin Expressed in Mitochondria
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Product Overview
Description
Human Umbilical Vein Endothelial Cells (HUVECs) are primary endothelial cells derived from the umbilical vein. When engineered to express aequorin targeted to mitochondria, they become a powerful model for studying real-time mitochondrial calcium dynamics in vascular endothelium.
Aequorin is a bioluminescent calcium-sensitive protein that emits light upon binding Ca², allowing precise measurement of mitochondrial calcium flux without external dyes.
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Key Features
Catalog Number: NBP-0001-AEQ-Mito
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
Characteristics:
-Primary endothelial phenotype (non-immortalized, physiologically relevant)
-Form cobblestone monolayers in culture
-Express endothelial markers such as VE-cadherin, CD31, and vWF
-Responsive to shear stress and inflammatory stimuli
-Mitochondrial-targeted aequorin expression enables:
-Direct monitoring of mitochondrial Ca² uptake
-High sensitivity calcium detection
-Minimal phototoxicity (bioluminescence-based)
Quick Facts
Product Type
Engineered Human Endothelial Cells
Viability
>95% viable (Trypan Blue exclusion)
Applications
-Mitochondrial calcium signaling studies -Vascul…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
-Mitochondrial calcium signaling studies -Vascular physiology and endothelial metabolism research -Drug screening for cardiovascular and mitochondrial toxicity -Oxidative stress and apoptosis research -Angiogenesis and vascular remodeling studies -Signal transduction pathway analysis in endothelial cells
Suitable for -mitochondrial calcium signaling studies -vascular physiology and endothelial metabolism research -drug screening for cardiovascular and mitochondrial toxicity -oxidative stress and apoptosis research -angiogenesis and vascular remodeling studies -signal transduction pathway analysis in endothelial cells studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
Support & Resources
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