Human Dermal Lymphatic Microvascular Endothelial Cells
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Description
Human Dermal Lymphatic Microvascular Endothelial Cells (HDLMECs) are the primary cellular constituents of the dermal lymphatic microvasculature, forming the thin-walled capillaries that serve as the primary entry points for interstitial fluid, immune cells, and macromolecules into the lymphatic system. As specialized endothelial cells lining the lymphatic capillaries of the skin, HDLMECs play a central role in tissue fluid homeostasis, immune surveillance, lipid absorption, and the regulation of inflammatory responses.
HDLMECs are morphologically and functionally distinct from both blood microvascular endothelial cells and endothelial cells of larger collecting lymphatic vessels. These differences reflect the unique structural demands of lymphatic capillary function, including high permeability, anchoring to the extracellular matrix, and the capacity to regulate immune cell trafficking.
Their distinctive molecular identity and accessibility from dermal tissue have made HDLMECs indispensable tools in both basic and translational research.
HDLMECs are isolated from lymphatic capillaries of normal human dermis, including juvenile foreskin and adult skin from various anatomical locations. Pure populations are obtained by immunomagnetic purification using a combination of lymphatic-specific markers, including LYVE-1, CD31, and CD34 differential expression, allowing the reliable separation of lymphatic from blood microvascular endothelial cells present in the same dermal tissue.
Guarantee expansion for a minimum of 2-5 population
doublings.
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Key Features
Catalog Number: NBP-0003
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
CELL CHARACTERISTICS
- Structural and Morphological Features:
HDLMECs possess several distinctive structural features that set them apart from blood microvascular endothelial cells:
Absent or poorly developed basement membranes: Unlike blood capillary endothelium, lymphatic capillaries have thin and discontinuous or absent basement membranes, which greatly enhances their permeability to macromolecules and cells.
-Molecular Markers
HDLMECs express a characteristic set of molecular markers that confirm their lymphatic identity and distinguish them from blood vascular endothelial cells:
LYVE-1 (Lymphatic Vessel Endothelial Hyaluronan Receptor-1): A hyaluronan receptor selectively expressed in lymphatic vessels in most tissues, LYVE-1 is the primary marker used for immunomagnetic isolation of HDLMECs and is a defining feature of the lymphatic endothelial phenotype.
Podoplanin (PDPN): A mucin-type transmembrane glycoprotein highly expressed in all lymphatic endothelial cell types. Podoplanin plays a role in lymphatico-venous separation during development and is used as a target for collecting and sorting lymphatic endothelial cells for in vitro culture.
Prox1 (Prospero Homeobox Protein 1): The master transcription factor of lymphatic endothelial cell fate and identity. Prox1 is essential for the specification and maintenance of the lymphatic endothelial phenotype; its loss leads to dedifferentiation of lymphatic endothelial cells toward a blood vascular endothelial phenotype.
Quick Facts
Product Type
Human Endothelial cells
Viability
>98% viable (Trypan Blue exclusion)
Applications
-In Vitro Lymphangiogenesis Modeling HDLMECs a…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
-In Vitro Lymphangiogenesis Modeling HDLMECs are the gold standard cell type for constructing in vitro models of lymphangiogenesis. Their robust response to VEGF-C via VEGFR-3 and their capacity to form tube-like structures in 3D matrix environments make them ideal for studying the mechanisms of lymphatic vessel growth and remodeling. These models are used to: Study the molecular regulators of lymphatic sprouting
Suitable for -in vitro lymphangiogenesis modeling hdlmecs are the gold standard cell type for constructing in vitro models of lymphangiogenesis. their robust response to vegf-c via vegfr-3 and their capacity to form tube-like structures in 3d matrix environments make them ideal for studying the mechanisms of lymphatic vessel growth and remodeling. these models are used to: study the molecular regulators of lymphatic sprouting studies and research applications
proliferation
Suitable for proliferation studies and research applications
and tube formation. Evaluate the pro- and anti-lymphangiogenic effects of growth factors
Suitable for and tube formation. evaluate the pro- and anti-lymphangiogenic effects of growth factors studies and research applications
cytokines
Suitable for cytokines studies and research applications
and pharmacological agents. Assess the role of extracellular matrix components in supporting or inhibiting lymphangiogenesis. -Tumor Lymphangiogenesis and Metastasis Research The lymphatic system is a major route for the dissemination of solid tumors
Suitable for and pharmacological agents. assess the role of extracellular matrix components in supporting or inhibiting lymphangiogenesis. -tumor lymphangiogenesis and metastasis research the lymphatic system is a major route for the dissemination of solid tumors studies and research applications
and HDLMECs are central to understanding this process. Lymphatic vessel density in and around tumors is closely associated with lymph node metastasis and patient prognosis. HDLMECs are used to: Model tumor-induced lymphangiogenesis: Tumor cells and tumor-associated macrophages secrete VEGF-C and VEGF-D
Suitable for and hdlmecs are central to understanding this process. lymphatic vessel density in and around tumors is closely associated with lymph node metastasis and patient prognosis. hdlmecs are used to: model tumor-induced lymphangiogenesis: tumor cells and tumor-associated macrophages secrete vegf-c and vegf-d studies and research applications
which drive the formation of new lymphatic vessels around tumors. HDLMECs are used to recapitulate this process in vitro. Study lymphatic metastasis mechanisms: Tumor cells must interact with and penetrate HDLMEC junctions to enter the lymphatic vasculature. Co-culture models using HDLMECs and cancer cell lines are used to dissect the molecular events governing this process. Investigate immunomodulatory roles: Beyond serving as passive conduits
Suitable for which drive the formation of new lymphatic vessels around tumors. hdlmecs are used to recapitulate this process in vitro. study lymphatic metastasis mechanisms: tumor cells must interact with and penetrate hdlmec junctions to enter the lymphatic vasculature. co-culture models using hdlmecs and cancer cell lines are used to dissect the molecular events governing this process. investigate immunomodulatory roles: beyond serving as passive conduits studies and research applications
HDLMECs in the tumor microenvironment can actively modulate anti-tumor immune responses
Suitable for hdlmecs in the tumor microenvironment can actively modulate anti-tumor immune responses studies and research applications
and these functions are studied using primary HDLMEC cultures. -Lymphedema and Lymphatic Disease Research Dysfunction or absence of lymphatic vessels leads to lymphedema
Suitable for and these functions are studied using primary hdlmec cultures. -lymphedema and lymphatic disease research dysfunction or absence of lymphatic vessels leads to lymphedema studies and research applications
a chronic and currently incurable condition characterized by debilitating tissue swelling and recurrent infections. HDLMECs are instrumental in studying the cellular and molecular basis of lymphatic diseases: Primary lymphedema: Missense mutations in the tyrosine kinase domain of VEGFR-3 are responsible for approximately 70% of cases of autosomal dominant primary congenital lymphedema (Milroy disease). HDLMECs provide a human-relevant platform for studying how these mutations impair VEGFR-3 signaling and lymphatic function. Secondary lymphedema: HDLMECs are used to study the mechanisms of lymphatic regeneration and the failure of lymphangiogenesis in post-surgical and post-radiation lymphedema
Suitable for a chronic and currently incurable condition characterized by debilitating tissue swelling and recurrent infections. hdlmecs are instrumental in studying the cellular and molecular basis of lymphatic diseases: primary lymphedema: missense mutations in the tyrosine kinase domain of vegfr-3 are responsible for approximately 70% of cases of autosomal dominant primary congenital lymphedema (milroy disease). hdlmecs provide a human-relevant platform for studying how these mutations impair vegfr-3 signaling and lymphatic function. secondary lymphedema: hdlmecs are used to study the mechanisms of lymphatic regeneration and the failure of lymphangiogenesis in post-surgical and post-radiation lymphedema studies and research applications
including after lymph node dissection in breast cancer treatment. Therapeutic lymphangiogenesis: HDLMECs are used to evaluate strategies for stimulating lymphatic vessel growth as a therapeutic approach to lymphedema
Suitable for including after lymph node dissection in breast cancer treatment. therapeutic lymphangiogenesis: hdlmecs are used to evaluate strategies for stimulating lymphatic vessel growth as a therapeutic approach to lymphedema studies and research applications
including VEGF-C gene therapy and cell-based regenerative approaches. - Inflammatory and Skin Disease Research Dysfunctional lymphatics are associated with chronic inflammatory and autoimmune diseases
Suitable for including vegf-c gene therapy and cell-based regenerative approaches. - inflammatory and skin disease research dysfunctional lymphatics are associated with chronic inflammatory and autoimmune diseases studies and research applications
including psoriasis and rheumatoid arthritis. HDLMECs serve as a model for: Inflammatory lymphangiogenesis: Under inflammatory conditions
Suitable for including psoriasis and rheumatoid arthritis. hdlmecs serve as a model for: inflammatory lymphangiogenesis: under inflammatory conditions studies and research applications
lymphatic vessels proliferate to facilitate immune cell clearance and the resolution of inflammation. HDLMECs are used to study how pro-inflammatory mediators such as TNF-
Suitable for lymphatic vessels proliferate to facilitate immune cell clearance and the resolution of inflammation. hdlmecs are used to study how pro-inflammatory mediators such as tnf- studies and research applications
IL-1
Suitable for il-1 studies and research applications
and NF-B regulate lymphatic endothelial function. Cutaneous immune surveillance: The dermal lymphatic network is a primary conduit for dendritic cell migration and antigen transport to lymph nodes. HDLMEC models are used to study how skin inflammation alters this process. Wound healing: HDLMECs are used to investigate the role of lymphangiogenesis in wound healing and tissue repair
Suitable for and nf-b regulate lymphatic endothelial function. cutaneous immune surveillance: the dermal lymphatic network is a primary conduit for dendritic cell migration and antigen transport to lymph nodes. hdlmec models are used to study how skin inflammation alters this process. wound healing: hdlmecs are used to investigate the role of lymphangiogenesis in wound healing and tissue repair studies and research applications
where restoration of lymphatic drainage is critical for the resolution of edema and inflammation.
Suitable for where restoration of lymphatic drainage is critical for the resolution of edema and inflammation. studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
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