Human PECAM1 shRNA Stable Transfected HUVECs
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Description
Human PECAM1 shRNA Stable Transfected Human Umbilical Vein Endothelial Cells (HUVECs) are genetically modified endothelial cells in which the expression of PECAM1 (CD31) has been stably reduced through short hairpin RNA (shRNA)-mediated gene silencing. These cells maintain long-term knockdown of PECAM1, making them a valuable model for studying endothelial cell biology, vascular integrity, leukocyte transmigration, angiogenesis, and cell-cell adhesion mechanisms.
Guarantee expansion for a minimum of 2-5 population
doublings.
IMPORTANT: Quality warranty requires use of NeoBioPharma media. Biohazardous material despite negative pathogen testing. Quality claims must be reported within 1 month.
Key Features
Catalog Number: NBP-0001siRNAPECAM1
Product Format: Frozen
Vial Cell Number: > 5x10[5] cells/vial
Passage:1(P1)
Storage: Liquid Nitrogen, Vapor Phase
Intended Use: Research use only (RUO)
CELL CHARACTERISTICS
-Cell Type: Primary Human Umbilical Vein Endothelial Cells (HUVECs)
-Genetic Modification: Stable integration of shRNA targeting PECAM1/CD31
-Target Gene: PECAM1 (CD31)
-Phenotype: Reduced PECAM1 protein and mRNA expression
-Morphology: Typical cobblestone-like endothelial morphology
-Endothelial Markers: Expression of endothelial markers such as VE-Cadherin, vWF, and eNOS (depending on knockdown efficiency)
-Growth Characteristics: Similar to parental HUVECs, with potential alterations in migration, adhesion, and barrier function
-Selection Marker: Usually maintained under antibiotic selection (e.g., puromycin) depending on vector design
-Applications: Suitable for long-term functional studies involving PECAM1 signaling pathways
Quick Facts
Product Type
Human Endothelial cells
Viability
>95% viable (Trypan Blue exclusion)
Applications
1. Vascular Biology Research Investigating the r…
Availability
In Stock • Global Shipping
Technical Specifications
Primary Applications
Applications & Use Cases
Primary Applications
1. Vascular Biology Research Investigating the role of PECAM1 in endothelial junction formation Studying vascular permeability and endothelial barrier integrity Understanding mechanotransduction in blood vessels 2. Angiogenesis Studies Evaluating the contribution of PECAM1 to capillary-like tube formation Examining endothelial migration during neovascularization Assessing angiogenic signaling pathways 3. Inflammation and Immune Cell Trafficking Modeling leukocyte-endothelial interactions Investigating transendothelial migration of immune cells Studying inflammatory responses and cytokine signaling 4. Cardiovascular Disease Research Exploring endothelial dysfunction associated with vascular disorders Analyzing mechanisms involved in atherosclerosis and thrombosis Evaluating therapeutic strategies targeting endothelial adhesion molecules 5. Drug Discovery and Screening Screening compounds that regulate endothelial adhesion and permeability Testing anti-inflammatory and anti-angiogenic therapeutics Identifying pathways affected by PECAM1 suppression
Suitable for 1. vascular biology research investigating the role of pecam1 in endothelial junction formation studying vascular permeability and endothelial barrier integrity understanding mechanotransduction in blood vessels 2. angiogenesis studies evaluating the contribution of pecam1 to capillary-like tube formation examining endothelial migration during neovascularization assessing angiogenic signaling pathways 3. inflammation and immune cell trafficking modeling leukocyte-endothelial interactions investigating transendothelial migration of immune cells studying inflammatory responses and cytokine signaling 4. cardiovascular disease research exploring endothelial dysfunction associated with vascular disorders analyzing mechanisms involved in atherosclerosis and thrombosis evaluating therapeutic strategies targeting endothelial adhesion molecules 5. drug discovery and screening screening compounds that regulate endothelial adhesion and permeability testing anti-inflammatory and anti-angiogenic therapeutics identifying pathways affected by pecam1 suppression studies and research applications
Research Areas
In Vitro Studies
Cell culture and screening applications
Molecular Biology
Gene expression & signaling studies
Drug Discovery
Screening & validation studies
Disease Modeling
Pathophysiology research
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